Mobilization of pseudomonas putida in kaolin soil by electrokinetic bioremediation technique

摘要

Contaminated soils are due to several factors which are caused by the removal of toxins,waste from industrial activities, heavy metals from landfill and organic and inorganic waste fromfertilizer used by farmers. This study aims to measure the mobilization of Pseudomonas putida cellsthrough kaolin clay soil using elctrokinetic bioremediation technique. The electrokinetic is used togenerate currents into the soil, thus migrating ions to opposite charge of electrodes. ThePseudomonas putida and distilled water was supplied into the anode and cathode reservoirs,respectively. The electrokinetic bioremediation testing was conducted for 5 days duration using 50V of electric current. The bacterial counts of Pseudomonas sp. are enumerated high at 1.3x107cfu/gww (near the anode), 5.0x106 cfu/gww (in the middle) and 8.0x106 cfu/gww (near the cathode).These results showed high survivability of Pseudomonas sp. until Day 5. It was demonstrated thatthe electrokinetic bioremediation technique can be used for Pseudomonas putida transportation inkaolin clay soil throughout the soil specimen and moving in same direction with the electroosmoticflow in electrokinetic bioremediation system